Inverted fluorescence microscope MF52-N promotes the development of microinjection in Shenzhen Unive
release time:2020-07-04

Inverted fluorescence microscope MF52-N to Shenzhen University to demonstrate the bright field and fluorescence imaging function of this microscope to customers on site, and set up an injection platform to test the imaging performance of this microscope in the field of microinjection.

The microinjection method is to inject foreign gene fragments into prokaryotic embryos or cultured cells directly by using a glass microinjection needle with a very thin tip (0.1-0.5 μ m), and then insert foreign genes into the host chromosome by the possible recombination, deletion, replication or translocation of the host genome sequence. The procedure of this kind of microinjection requires very precise micromanipulation equipment. When the long tip is made, a micro pipette stretcher is needed. When the injection is made, a micro manipulator is needed to fix the position of the tip. The advantage of this technique is that any DNA can be introduced into any kind of cell in principle. This method has been successfully applied to transgenic animals including mice, fish, rats, rabbits and many large livestock, such as cattle, sheep and pigs.

倒置荧光显微镜MF52在深圳大学推动显微注射法发展1.jpg   倒置荧光显微镜MF52在深圳大学推动显微注射法发展2.jpg   倒置荧光显微镜MF52在深圳大学推动显微注射法发展3.jpg

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