Upgrading Olympus CKX41 to fluorescence

Introduction of Olympus CKX41

The CKX41 is an inverted microscope suitable for regular cell observation including GFP and other fluorescence applications. Olympus launched it some years ago and has a wide range of users, Now it has been discontinued and replaced by Olympus CKX53.

The original Olympus CKX41 fluorescence configuration is 50W mercury illumination, and the package has been discontinued. Users are looking for alternative fluorescence upgrading solutions.

Olympus CKX41 inverted phase contrast microscope
Olympus CKX41 mercury fluorescence configuration

Olympus CKX41 inverted phase contrast configuration

Olympus CKX41 mercury fluorescence configuration

How do we upgrade the Olympus CKX41 to fluorescence?

A third-party fluorescence module in an LED light source is a good replacement for a mercury fluorescence illuminating system. There are advantages even compared to the original mercury fluorescence illuminating.

To install the LED module to the CKX41, no structure or components need to be modified or added, it is a whole package with all necessary accessories. Users can finish the installation easily by watching the installation video or user manual.

There are installtion photos at customer side to show the well done system.

Olympus CKX41 with MI-LED fluorescence module
Installation of MI-LED green channel with Olympus CKX41

Performance photos of BYU channels

GFP stain cells in green fluorescence
Green channel fluorescence of Olympus CKX41
UV excited stained cell 10X objective

What is the LED fluorescence module for an inverted microscope?

The MIF-LED fluorescence module is designed to upgrade the Olympus inverted biological microscope to fluorescence function. Special with built-in color LED lamps and fluorescence filters offering BGUR four channels and a bright field lighting channel.

Features:

■ Compact design contains light sources and filters in one unit.
■ Instant on-off, no need to wait for pre-heating or cooling.
■ Freely select fluorescence bands and quantities according to needs.
■ Fluorescence channel and brightness display function, display working status.
■ Brightness memory function, can remember the last used brightness.
■ Digital screen shows light intensity 0~100%
■ Light source synchronous switching with filter groups.
■ No requirement for external or added power supply.
■ CE, FCC, EMC, EU, ISO certified.

Standard configuration
ModelLED lampFilter wavelengthMainly Applied Fluorochrome
Excitation filterDichroic mirrorEmission filter
BBlue475/30nm>505nm530/40nmGFP / FITC /EGFP/ Malaria diagnostic/ Alexa 488 / Cy2@ / Fluo-4 / FluorX@ / Fluoro-Jade
GGreen530/40nm>570nm575nmLPPI / EB / EH /TRITC
YYellow560/40nm>600nm610nmLPmCherry / Texas Red /     AlexaFluor 594
UUV375/30nm>415nm460/50nmDAPI / Hoechst 33342&33258 / AMCA/AMCA-X / Alexa 350
Remark*Olympus CX41 UV excitation only can be long-pass filter
Light sourceBlue &UV: 3W LED cold lamp for each filter group
Green/Yellow: 5W LED cold lamp for each filter group
ObservationFluorescence
Bright field & phase contrast by microscope original lighting
OperationLever : B, G, UV/O
Power controlRota-table knob, continuously adjustable brightness
ScreenDigital screen to show light intensity 0~100%, rememer light intensity of each color
Input powerDC 12V 2A
ShellHigh rigid precision-cast aluminium with coating and vents