Respiratory Infection Microscopy Diagnosis
With the development of new test items in the laboratory, the respiratory tract nine joint inspection is popular in more and more hospitals. The package combination makes the detection of clinical respiratory tract infections more efficient, specific, and comprehensive. A fluorescence microscope is the equipment to process its quick diagnosis in the pathology unit.
What is the joint inspection of respiratory tract?
Respiratory infection is mainly caused by bacteria, microorganisms, and fungi, the joint tests of the respiratory tract are also called combined tests of IgM antibodies against nine respiratory pathogens, in China mainly nine joint tests which can test nine kinds of infection resources in one slide.
- Legionella pneumophila serogroup 1 IgM antibody
- Mycoplasma pneumoniae IgM antibody
- Rickettsia Q IgM antibody
- Chlamydia pneumoniae IgM antibody
- Adenovirus IgM antibody
- Respiratory syncytial virus IgM antibody
- Influenza A virus IgM antibody
- Influenza B virus IgM antibody
- Parainfluenza virus 1, 2 and 3 IgM antibody)
The indirect immunofluorescence method was used to detect IgM of nine pathogens. Because the body is stimulated by antigen, the first antibody produced is IgM, but the half-life of IgM is short (about 5 days). If the serum-specific IgM antibody content increases, it indicates that there is a recent infection, which is helpful in the early diagnosis of infectious diseases.
Map of respiratory track negative results
Map of respiratory track positive results
What kind of microscope is used to diagnose?
A routine fluorescence microscope in LED lighting is advised, take an example of the normal uses of diagnosis fluorescence reagents manufactured by VIRCELL MICROBIOLOGISTS in Spain. The reagent is mostly effectively excited under Blue light EX460~480nm and Green light EX510~550nm.
VIRCELL sample images taken with MSHOT microscope
Recommend LED fluorescence microscope configuration
MF31 series fluorescence microscope adopting excellent quality infinity optical system combined with LED fluorescence illuminator. The LED fluorescence system is modular design, does not need external power box, it can offer blue, green and UV fluorescence excitation groups. Digital screen shows and remember each fluorescence channel light intensity.
| Name | Blue | Green | UV |
| Excitation filter | 460-490nm | 510-550nm | 330-380nm |
| Dichroic mirror | 500nm | 570nm | 400nm |
| Emission filter | 510nmLP | 590nmLP | 420nmLP |
- Instant on-off is convenient for clinical workers
- Low voltage is safe and saves energy
- Memory the last setting brightness highly improves efficiency when moving channels
- Light baffle to protect user’s eye from light
- The LED fluorescence module is pre-adjusting, just put on to use
