How do we upgrade the Nikon Eclipse Si microscope to reflect fluorescence?
Nikon has designed the ECLIPSE Si to focus on comfort for users who spend hours using the microscopes for a long time. Using optional accessories, the ECLIPSE Si allows for a wide variety of observation methods in addition to brightfield, such as phase contrast, dark field, polarizing, and diascopic fluorescence.
What is diascopic fluorescence? By simply inserting an EX filter slider into the condenser slot and a BA filter slider into the nosepiece slot, fluorescence observation of specimens expressing GFP or stained with fluorescent dyes such as FITC and Alexa 488 can be performed.
Reflected fluorescence upgrading
Solution of epi-illuminating fluorescence
The LED fluorescence module is specially designed for epi-fluorescence observation. By inserting the module between the microscope observation head and the body, independent observation of each multi-color fluorescence channel can be achieved. It not only supports green fluorescence expression, but also supports red fluorescence expression such as PI, mCherry, TexasRed, Cy3, Cy5, and blue fluorescence expression such as DAPI and Calco fluo.
What is an LED fluorescence module?
A modularly designed attachment for fluorescence microscope to excited specimen and express fluorescence signal. It contains the light source and fluorescence filters. You don’t need any extra accessories.
- Right photo is the installation photo of Nikon Eclipse Si microscope with MF-BGU-LED fluorescence module
Fluorescence performance photos were taken through the eyepiece with a Nikon Eclipse Ci / Si microscope with LED fluorescence module
Features of the LED fluorescence module
- Various channels of BGYUR excitation lighting from 330nm to 625nm for various fluorescence dyes.
- Besides the single-channel model, a multi-channel model of 2~4 channels in one unit is available.
- A digital screen showing the light intensity of each channel by 0~100% works for fluorescence quantification.
- Independent brightness memory function, each fluorescence channel can independently set the brightness and record the set brightness by default.
| LED Fluorescence Module Standard specification | |||||
| Model | Filter | Filter wavelength | Recommend Application | ||
| Excitation | Dichroic mirror | Emission | |||
| B | Blue | 475/30nm | >505nm | 530/40nm | GFP / FITC /EGFP/ Malaria diagnostic/ Alexa 488 / Cy2@ / Fluo-4 / FluorX@ / Fluoro-Jade |
| Y | Yellow | 560/40nm | >600nm | 610nmLP | mCherry / Texas Red / AlexaFluor 594 |
| U | UV | 355/50nm | >410nm | 420nmLP | DAPI / Hoechst 33342&33258 / AMCA/AMCA-X / Alexa 350 |
| G | Green | 530/40nm | >570nm | 575nmLP | PI / EB / EH /TRITC |
| Light source | Blue &UV: 3W LED cold lamp for each filter group Green/Yellow: 5W LED cold lamp for each filter group | ||||
| Observation | Fluorescence | ||||
| Bright field & phase contrast by microscope original lighting | |||||
| Digital screen | Show light intensity 0~100% and remember each color light source brightness | ||||
| Operation | Lever : B, G, UV/O (bright field) | ||||
| Power control | Rota-table knob, continuously adjustable brightness | ||||
| Input power | 12V 2A | ||||
| Shell | High rigid precision-cast aluminium with coating | ||||
| Light baffle | Orange color plastic light baffle | ||||