How do we upgrade Olympus IX73 to fluorescence?
Introduction of the Olympus IX3 series microscope
The IX3 is a highly expandable platform for live cell imaging designed with the scientist’s workflow in mind.
The Olympus IX3 microscope system offers diverse units, bringing greater efficiency to everything from casual observation to serious imaging. Simple cassette-like insertion into the deck makes it easy to mount fluorescence modules, fluorescence illuminators, and other desired units.
The IX3 framework for Olympus IX53, IX73, and IX83.
A third party LED fluorescence module and illuminator
What is the cost-effective fluorescence upgrading solution?
Based on the flexible expansion feature of the IX3 frame, MICRO-SHOT has launched a modular fluorescence module. By adding this fluorescence module, the fluorescence observation function can be realized without changing the original structure of the IX3. The fluorescence filter and light source have been integrated, and no additional accessories are needed.
Installation photo of Olympus IX73 with MIF-LED fluorescence module
Performance photos of GFP/EGFP and DAPI stained live cell under IX73 microscope with MSHOT LED fluorescence module
Introduction of the fluorescence module
The conventional fluorescence excitation bands can be selected from blue, green, yellow, ultraviolet and red excitation, and the module can support three different fluorescence channels at a time. Another bright field channel is reserved for microscope transmission bright field illumination.
Suitable for common dyes for living cell observation GFP, Alexas Fluor, PI, TexasRed, mCherry, Cy3, Cy5, DAPI, etc.
| Standard configuration | |||||
| Model | LED lamp | Filter wavelength | Mainly Applied Fluorochrome | ||
| Excitation filter | Dichroic mirror | Emission filter | |||
| B | Blue | 475/30nm | >505nm | 530/40nm | GFP / FITC /EGFP/ Malaria diagnostic/ Alexa 488 / Cy2@ / Fluo-4 / FluorX@ / Fluoro-Jade |
| G | Green | 530/40nm | >570nm | 575nmLP | PI / EB / EH /TRITC |
| Y | Yellow | 560/40nm | >600nm | 610nmLP | mCherry / Texas Red / AlexaFluor 594 |
| U | UV | 375/30nm | >415nm | 460/50nm | DAPI / Hoechst 33342&33258 / AMCA/AMCA-X / Alexa 350 |